CHEMGRID MOLECULE CARD

AMINOPTERIN

AMINOPTERIN in ChemGrid: 192 screened assays, 1 with significant responses. Explore chemical identifiers, strain activity and target evidence.

SPE01500679

Explore activity & SONAR

Chemical structure of AMINOPTERIN — Stored molecular structure

192Screened assays

1Significant assay responses

9Source-labelled target records

Identity and chemistry

ChemGrid identifier: SPE01500679
Molecular formula: C19H20N8O5
InChIKey: TVZGACDUOSZQKY-UHFFFAOYSA-N
Libraries: Spectrum, SPECMTS3
Other recorded names: N-(4-(((2,4-Diamino-6-pteridinyl)methyl)amino)benzoyl)glutamic acid

Stored SMILES

Nc1nc(N)c2nc(CNc3ccc(cc3)C(=O)NC(CCC(O)=O)C(O)=O)cnc2n1

Matched external records: PubChem 2154

Chemical identifiers and library names are reproduced from ChemGrid records. Missing values are omitted.

Measured chemical–genetic activity

192 measurements across 192 distinct library–species–strain assays; 1 assays have at least one significant response (|Z| ≥ 4, p ≤ 0.001, excluding flagged outliers).

Responses are indirect screening phenotypes. They do not establish that a strain gene is a direct molecular target. Strains and timepoints are retained as recorded; these counts are not counts of unique ORFs.

Up to 12 strongest recorded single-strain responses by absolute Z-score
Strain	Library	Species identifier	Z-score
FUR4	Spectrum	4932	4.96

Spectrum compound-combination identifiers are excluded from this short table. The interactive profile retains the full measurements.

Recorded target evidence and yeast matches

Source annotations are shown separately from SONAR hypotheses. Evidence type and identity matching can vary; consult the linked source and interactive evidence panel.

Target	Source	Yeast ORF / orthologue
Bifunctional dihydrofolate reductase-thymidylate synthase	ChEMBL analogue	No stored yeast mapping
NCOA3 · Nuclear receptor coactivator 3	ChEMBL analogue	No stored yeast mapping
NFE2L2 · Nuclear factor erythroid 2-related factor 2	ChEMBL analogue	No stored yeast mapping
POLI · DNA polymerase iota	ChEMBL analogue	No stored yeast mapping
PTR1 · Pteridine reductase 1	ChEMBL analogue	No stored yeast mapping
SMN1 · Survival motor neuron protein	ChEMBL analogue	No stored yeast mapping
Tb08.26N11.790 · Pteridine reductase, putative	ChEMBL analogue	No stored yeast mapping
TP53 · Cellular tumor antigen p53	ChEMBL analogue	No stored yeast mapping
Txnrd1 · Thioredoxin reductase 1, cytoplasmic	ChEMBL analogue	TRR1 YDR353W (ortholog_many2many); TRR2 YHR106W (ortholog_many2many)

Structural activity predictions and SONAR

Stored structural activity predictions are available in the interactive profile, with assay-specific cutoffs and applicability-domain assessments.

SONAR ranks candidate targets through genetic and physical interaction evidence. Structural SONAR uses predicted strain responses; measured SONAR uses experimental responses. These are experimental hypotheses, not confirmed binding targets. Outside-domain predictions are treated as unknown.

Open the complete AMINOPTERIN research profile →

Sources and provenance

Screening measurements: ChemGrid chemical-genetic datasets. Wildenhain et al., Cell Systems (2015). Target annotations: sources named in the evidence table. Structure and library metadata: stored ChemGrid catalogue records.

Card snapshot: 2026-11-02. Linked interactive views may contain newer data.