CHEMGRID MOLECULE CARD

PYRIMETHAMINE

PYRIMETHAMINE in ChemGrid: 205 screened assays, 2 with significant responses. Explore chemical identifiers, strain activity and target evidence.

SPE01500520

Explore activity & SONAR

Chemical structure of PYRIMETHAMINE — Stored molecular structure

205Screened assays

2Significant assay responses

4Source-labelled target records

Identity and chemistry

ChemGrid identifier: SPE01500520
Molecular formula: C12ClH13N4
CAS registry number: 58-14-0
InChIKey: WKSAUQYGYAYLPV-UHFFFAOYSA-N
Libraries: Spectrum, SPECMTS3, Spectrum_ED
Other recorded names: Pyrimethamine

Stored SMILES

CCc1nc(N)nc(N)c1c1ccc(Cl)cc1

Matched external records: PubChem 4993

Chemical identifiers and library names are reproduced from ChemGrid records. Missing values are omitted.

Measured chemical–genetic activity

205 measurements across 205 distinct library–species–strain assays; 2 assays have at least one significant response (|Z| ≥ 4, p ≤ 0.001, excluding flagged outliers).

Responses are indirect screening phenotypes. They do not establish that a strain gene is a direct molecular target. Strains and timepoints are retained as recorded; these counts are not counts of unique ORFs.

Up to 12 strongest recorded single-strain responses by absolute Z-score
Strain	Library	Species identifier	Z-score
gcn3	SPECMTS3	4932	9.86
doa4	SPECMTS3	4932	-4.06

Spectrum compound-combination identifiers are excluded from this short table. The interactive profile retains the full measurements.

Recorded target evidence and yeast matches

Source annotations are shown separately from SONAR hypotheses. Evidence type and identity matching can vary; consult the linked source and interactive evidence panel.

Target	Source	Yeast ORF / orthologue
Bifunctional dihydrofolate reductase-thymidylate synthase	ChEMBL	No stored yeast mapping
DHFR · Dihydrofolate reductase	ChEMBL	DFR1 YOR236W (ortholog_one2many)
Bifunctional dihydrofolate reductase-thymidylate synthase	ChEMBL analogue	No stored yeast mapping
DHFR · Dihydrofolate reductase	ChEMBL analogue	DFR1 YOR236W (ortholog_one2many)

Structural activity predictions and SONAR

Stored structural activity predictions are available in the interactive profile, with assay-specific cutoffs and applicability-domain assessments.

SONAR ranks candidate targets through genetic and physical interaction evidence. Structural SONAR uses predicted strain responses; measured SONAR uses experimental responses. These are experimental hypotheses, not confirmed binding targets. Outside-domain predictions are treated as unknown.

Open the complete PYRIMETHAMINE research profile →

Sources and provenance

Screening measurements: ChemGrid chemical-genetic datasets. Wildenhain et al., Cell Systems (2015). Target annotations: sources named in the evidence table. Structure and library metadata: stored ChemGrid catalogue records.

Card snapshot: 2026-11-02. Linked interactive views may contain newer data.