CHEMGRID MOLECULE CARD

Hydroxyurea

Hydroxyurea in ChemGrid: 24 screened assays, 1 with significant responses. Explore chemical identifiers, strain activity and target evidence.

LOPAC 00959

Explore activity & SONAR

Chemical structure of Hydroxyurea — Stored molecular structure

24Screened assays

1Significant assay responses

5Source-labelled target records

Identity and chemistry

ChemGrid identifier: LOPAC 00959
Molecular formula: CH4N2O2
InChIKey: VSNHCAURESNICA-UHFFFAOYSA-N
Libraries: Lopac

Stored SMILES

NC(=O)NO

Matched external records: PubChem 3657

Chemical identifiers and library names are reproduced from ChemGrid records. Missing values are omitted.

Measured chemical–genetic activity

24 measurements across 24 distinct library–species–strain assays; 1 assays have at least one significant response (|Z| ≥ 4, p ≤ 0.001, excluding flagged outliers).

Responses are indirect screening phenotypes. They do not establish that a strain gene is a direct molecular target. Strains and timepoints are retained as recorded; these counts are not counts of unique ORFs.

Up to 12 strongest recorded single-strain responses by absolute Z-score
Strain	Library	Species identifier	Z-score
Pac10	Lopac	4932	-26.34

Spectrum compound-combination identifiers are excluded from this short table. The interactive profile retains the full measurements.

Recorded target evidence and yeast matches

Source annotations are shown separately from SONAR hypotheses. Evidence type and identity matching can vary; consult the linked source and interactive evidence panel.

Target	Source	Yeast ORF / orthologue
EHMT2 · Histone-lysine N-methyltransferase EHMT2	ChEMBL	SET2 YJL168C (ortholog_many2many)
NFKB1 · Nuclear factor NF-kappa-B p105 subunit	ChEMBL	No stored yeast mapping
EHMT2 · Histone-lysine N-methyltransferase EHMT2	ChEMBL analogue	SET2 YJL168C (ortholog_many2many)
NFKB1 · Nuclear factor NF-kappa-B p105 subunit	ChEMBL analogue	No stored yeast mapping
Ribonucleoside reductase	ChemGrid bioactivity_info	No stored yeast mapping

Structural activity predictions and SONAR

Stored structural activity predictions are available in the interactive profile, with assay-specific cutoffs and applicability-domain assessments.

SONAR ranks candidate targets through genetic and physical interaction evidence. Structural SONAR uses predicted strain responses; measured SONAR uses experimental responses. These are experimental hypotheses, not confirmed binding targets. Outside-domain predictions are treated as unknown.

Open the complete Hydroxyurea research profile →

Sources and provenance

Screening measurements: ChemGrid chemical-genetic datasets. Wildenhain et al., Cell Systems (2015). Target annotations: sources named in the evidence table. Structure and library metadata: stored ChemGrid catalogue records.

Card snapshot: 2026-11-02. Linked interactive views may contain newer data.