4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
4932 |
YDL020C |
Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses |
Null mutant is viable, exhibits synthetic interactions with sen3, sun1, and cdc28-1N |
RPN4 |
4932 |
YER016W |
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally |
Null mutant is viable, causes cold sensitivity, benomyl supersensitivity, aberrant microtubule morphology. During mitosis in bim1 mutants, the nucleus fails to move to the mother-bud neck. |
BIM1 |
4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
4932 |
YGL086W |
Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p |
|
MAD1 |
4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
4932 |
YGR106C |
Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar memebrane |
|
YGR106C |
4932 |
YHR191C |
Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
|
CTF8 |
4932 |
YJL030W |
Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p |
|
MAD2 |
4932 |
YKL113C |
5- to 3- exonuclease, 5- flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family |
Null mutant demonstrates temperature-sensitive growth and sensitivity to UV light and methylmethane sulfonate. rad27 mutant cells are defective in Okazaki fragment maturation. |
RAD27 |
4932 |
YLR200W |
Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin |
|
YKE2 |
4932 |
YLR418C |
Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinct from Srb-containing Pol II complexes; required for expression of certain genes, modification of some histones, and telomere maintenance |
Mutations affect cell growth and the abundance of transcripts from a subset of genes |
CDC73 |
4932 |
YML032C |
Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis |
Null mutant is viable, radiation sensitive; rad52 rad27 double mutants are inviable, double strand break ends are excessively recessed in mutant, rad52 is rescued by rad50 spo13, but not spo13, and is classified as late recombination gene. Growth defects of mgs1 rad18 double mutants are suppressed by overexpression of Rad52.; Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies. |
RAD52 |
4932 |
YML094W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol; synthetically lethal with tub4-1 mutations |
GIM5 |
4932 |
YMR078C |
Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint |
Null mutant is viable, exhibits increased level of spontaneous mitotic recombination, slow growth, and cold sensitivity |
CTF18 |
4932 |
YMR224C |
Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved |
Null mutant is viable, methyl methanesulfonate-sensitive and displays hyper-recombination in mitosis. mre11 is rescued by spo13 and epistatic to rad50s, suggesting it is an early recombination function. |
MRE11 |
4932 |
YNL298W |
Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p |
Null mutant is viable, possesses a cytokinesis defect; cla4 cln1 cln2 strains are inviable; cla4 ste20 double deletion mutants cannot maintain septin rings at the bud neck and and cannot undergo cytokinesis |
CLA4 |
4932 |
YNL330C |
Histone deacetylase; regulates transcription and silencing |
Null mutant is viable and shows reduced potassium dependency, mating defects, hypersensitivity to cycloheximide, and constitutive derepression of acid phosphatase; mutant epistasis analysis indicates that RPD3 acts in the same pathway as UME4/SIN3; homozygous mutant diploid is defective in sporulation and recombination |
RPD3 |
4932 |
YNR052C |
RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3- to 5- mRNA deadenylation |
Mutant is resistant to glucose derepression, temperature-sensitive, and unable to sporulate and contains reduced amounts of reserve carbohydrates |
POP2 |
4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
4932 |
YOR026W |
Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p |
|
BUB3 |
4932 |
YOR349W |
Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl |
Null mutant is viable, exhibits cold sensitivity for viability; defect in nuclear migration and nuclear fusion, supersensitivity to benomyl and nocodozole |
CIN1 |
4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |