| 4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
| 4932 |
YAL024C |
Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP |
lethal at low temperature (8 degrees C) |
LTE1 |
| 4932 |
YBR010W |
One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation |
|
HHT1 |
| 4932 |
YBR136W |
Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination |
Null mutant is inviable; overproduction of Rad53p rescues some esr1 alleles |
MEC1 |
| 4932 |
YDR334W |
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Null mutant is viable and shows no growth defects; swr1 rat8-2 and swr1 rsc9-1double mutants has a slow growth phenotype; SWR1 is a partial High copy suppressor of pse1-1 kap123 |
SWR1 |
| 4932 |
YDR359C |
Component of the NuA4 histone acetyltransferase complex |
|
EAF1 |
| 4932 |
YDR369C |
Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling |
X-ray sensitive, spores inviable, xrs2 is rescued by spo13 and is epistatic to rad52 |
XRS2 |
| 4932 |
YEL003W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, sensitive to anti-microtubule drugs benomyl and nocadazole; synthetically lethal with tub4-1 mutations |
GIM4 |
| 4932 |
YEL061C |
Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation |
Null mutant is viable; cin8 dyn1 and cin8 kip1 double deletion mutants are inviable |
CIN8 |
| 4932 |
YER007W |
Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl |
|
PAC2 |
| 4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
| 4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
| 4932 |
YJL030W |
Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p |
|
MAD2 |
| 4932 |
YJL115W |
Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed |
|
ASF1 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YLR200W |
Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin |
|
YKE2 |
| 4932 |
YML041C |
Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS71 |
| 4932 |
YML094W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol; synthetically lethal with tub4-1 mutations |
GIM5 |
| 4932 |
YNL107W |
Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain |
|
YAF9 |
| 4932 |
YNL153C |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
|
GIM3 |
| 4932 |
YNL298W |
Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p |
Null mutant is viable, possesses a cytokinesis defect; cla4 cln1 cln2 strains are inviable; cla4 ste20 double deletion mutants cannot maintain septin rings at the bud neck and and cannot undergo cytokinesis |
CLA4 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YPL241C |
Tubulin folding factor C (putative) involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl |
Null mutant is viable but shows supersensitivity to benomyl and nocodazole, cold sensitivity, defects in karyogamy, and increased rates of chromosome loss; shows genetic interaction with tubulin mutations |
CIN2 |
| 4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |
