| 4932 |
YAR002W |
Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance |
|
NUP60 |
| 4932 |
YBR088C |
Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair |
|
POL30 |
| 4932 |
YDL020C |
Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses |
Null mutant is viable, exhibits synthetic interactions with sen3, sun1, and cdc28-1N |
RPN4 |
| 4932 |
YDL042C |
Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication |
Null mutant is viable; sir2 mutations suppress mitotic and meiotic intra- and interchromosomal rDNA recombination (10-15 fold). RAD52 and RAD50 are dispensable for basal level rDNA exchange in SIR2 but are required for increased exchange in sir2 |
SIR2 |
| 4932 |
YDL220C |
Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping |
|
CDC13 |
| 4932 |
YER016W |
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally |
Null mutant is viable, causes cold sensitivity, benomyl supersensitivity, aberrant microtubule morphology. During mitosis in bim1 mutants, the nucleus fails to move to the mother-bud neck. |
BIM1 |
| 4932 |
YIR002C |
Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype |
|
MPH1 |
| 4932 |
YJL115W |
Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed |
|
ASF1 |
| 4932 |
YJR043C |
Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p |
Null mutant is viable but is cold-sensitive, hydroxyurea-sensitive, defective for induced mutagenesis, synthetic lethal with pol3, pol30 and pol31 |
POL32 |
| 4932 |
YKL114C |
Major apurinic/apyrimidinic endonuclease, 3--repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3--5- exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine |
hypersensitive to both oxidative and alkylating agents that damage DNA; higher rate of spontaneous mutation |
APN1 |
| 4932 |
YKR056W |
tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair |
|
TRM2 |
| 4932 |
YKR082W |
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p |
Null mutant is viable but grows slowly and is temperature-sensitive; at nonpermissive temperature, poly(A)+ RNA accumulates in nucleus (although nuclear import of karyophilic proteins is not blocked) and nuclear pores cluster; synthetically lethal with nup120 |
NUP133 |
| 4932 |
YLR234W |
DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination |
Null mutant exhibits a genomic instability phenotype that includes slow growth, hyper-sensitivity to genotoxic agents, mitotic hyper-recombination, increased chromosome missegregation, and meiotic failure. top3 is RAD1-dependent hyper-Rec in mitosis, suggesting that top3 damage is channeled to the recombination repair pathway by RAD1; TOP3 is required for sporulation. |
TOP3 |
| 4932 |
YLR318W |
Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia |
Null mutant is viable, exhibits progressively shorter telomeres, cellular senescence and a telomerase-minus defect; est2 rad52 mutants are inviable |
EST2 |
| 4932 |
YLR320W |
Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks |
Null: Null phenotype in haploids of either mating type and diploid is extreme sensitivity to MMS or hydroxyurea, moderate sensitivity to gamma or UV irradiation. Diploid is very sensitive to camtothecin. Diploid is also sensitive to bleomycin.. |
MMS22 |
| 4932 |
YLR357W |
Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance |
|
RSC2 |
| 4932 |
YLR442C |
Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p |
sterile |
SIR3 |
| 4932 |
YML028W |
Ubiquitous housekeeping thioredoxin peroxidase, reduces reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; mediates redox regulation of the nuclear localization of Yap1p; deletion results in mutator phenotype |
Null mutant is viable, grows slower than wild-type under aerobic conditions |
TSA1 |
| 4932 |
YML061C |
DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA |
Mitochondrial DNA is heat-labile; abnormal telomere formation |
PIF1 |
| 4932 |
YML095C |
Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein |
radiation sensitive; Deletion of this nucleotide excision repair (NER) gene results in lower levels of cross-link-induced recombination but higher mutation frequencies than wild-type cells. |
RAD10 |
| 4932 |
YMR001C |
Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate |
Null mutant is inviable. cdc5(ts) mutants form synaptonemal complexes lacking central elements and arrest either at meiosis I with broken spindles or at meiosis II with short spindles. Late shifts to a restrictive temperature result in reductional dyads; each spore contains an entire meiosis II short spindle with unseparated chromatids. In some strains at semi-permissive temperature, chromosomes segregate reductionally or equationally depending upon the centromere. |
CDC5 |
| 4932 |
YMR038C |
Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation |
Null mutant is viable, methionine and lysine auxotroph, pH and temperature sensitive; sensitive to superoxide generating drugs and light irradiation, exhibits diminution of calcineurin activity |
CCS1 |
| 4932 |
YMR048W |
Protein required for accurate chromosome segregation during meiosis |
Null: missegregates chromosomes in meiosis |
CSM3 |
| 4932 |
YMR190C |
Nucleolar DNA helicase of the RecQ family involved in maintenance of genome integrity, regulates chromosome synapsis and meiotic crossing over; has similarity to human BLM and WRN helicases implicated in Bloom and Werner syndromes |
Null mutant is viable; strains lacking SGS1 exhibit elevated levels of chromosome misseggregation during both mitotic and meiotic division. sgs1 null strains suppress the slow growth of a top3 delta strain lacking topoisomerase III and show an increase in subtelomeric Y- instability due to hyperrecombination. |
SGS1 |
| 4932 |
YOL006C |
Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination |
|
TOP1 |
