| 4932 |
YDL074C |
E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain |
null mutant is sensitive to brefeldin A |
BRE1 |
| 4932 |
YDL099W |
Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes |
|
BUG1 |
| 4932 |
YDL100C |
ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress |
Null: YDL100c gene disruption results in sensitivity to As(III), As(V), Co(II) and Cu(II). |
GET3 |
| 4932 |
YDL192W |
ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p |
Null mutant is viable and shows slow growth, cold sensitivity and sensitivity to normally sublethal concentrations of fluoride ion in the medium. |
ARF1 |
| 4932 |
YDL226C |
ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p |
|
GCS1 |
| 4932 |
YDR108W |
Subunit of TRAPP (transport protein particle), a multi-subunit complex involved in targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment; protein has late meiotic role, following DNA replication |
|
GSG1 |
| 4932 |
YDR310C |
Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; involved in telomere maintenance, regulated by the pachytene checkpoint |
Restores silencing at HML and HMR in presence of sir2, sir3 and sir4 mutants |
SUM1 |
| 4932 |
YDR392W |
Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters |
Null mutant is viable, exhibits defects in mating and sporulation, Ty transcription, and suppression of certain Ty insertion mutations |
SPT3 |
| 4932 |
YDR414C |
Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) |
disruption of the retention system for ER proteins; defects in the Golgi-dependent modification of glycoproteins; exhibits defects in N-glycosylation of proteins |
ERD1 |
| 4932 |
YEL003W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, sensitive to anti-microtubule drugs benomyl and nocadazole; synthetically lethal with tub4-1 mutations |
GIM4 |
| 4932 |
YEL018W |
Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex |
|
EAF5 |
| 4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
| 4932 |
YER140W |
Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
|
YER140W |
| 4932 |
YGL043W |
General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites |
Null mutant is viable; reduced induction of DNA strand transfer; sensitivity to 6-azauracil |
DST1 |
| 4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
| 4932 |
YGL194C |
Histone deacetylase required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity |
|
HOS2 |
| 4932 |
YGL244W |
Subunit of the RNA polymerase II-associated Paf1 complex; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in telomere maintenance |
Null mutant is viable and can suppress TATA box-binding protein mutants (SPT15) in an allele-specific fashion |
RTF1 |
| 4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
| 4932 |
YHR078W |
High osmolarity-regulated gene of unknown function |
|
YHR078W |
| 4932 |
YIL039W |
Putative protein of unknown function; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) |
|
TED1 |
| 4932 |
YKR029C |
Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains |
|
SET3 |
| 4932 |
YLR039C |
Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes |
defective in the transcription of both ribosomal protein genes and ribosomal RNA |
RIC1 |
| 4932 |
YLR262C |
GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 |
Null mutant is viable, temperature sensitive; suppressed by ssd1 and imh1 mutations |
YPT6 |
| 4932 |
YLR418C |
Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinct from Srb-containing Pol II complexes; required for expression of certain genes, modification of some histones, and telomere maintenance |
Mutations affect cell growth and the abundance of transcripts from a subset of genes |
CDC73 |
| 4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
