4932 |
YBR164C |
Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
|
ARL1 |
4932 |
YDL074C |
E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain |
null mutant is sensitive to brefeldin A |
BRE1 |
4932 |
YDL220C |
Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping |
|
CDC13 |
4932 |
YDR126W |
Palmitoyltransferase that acts on the SNAREs Snc1p, Syn8p, Tlg1p and likely on all SNAREs; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion |
Profilin synthetic lethal |
SWF1 |
4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
4932 |
YER122C |
ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p |
|
GLO3 |
4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
4932 |
YGL054C |
Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon |
Null mutant is viable but exhibits defects in sporulation (diploids) and bud site selection (haploids). Null mutants also retain the bud site selection marker, Axl2p, in the ER and exhibit slow recovery from selective to rich media. |
ERV14 |
4932 |
YGR105W |
Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also cannot grow on glycerol or at pH 7.5 |
VMA21 |
4932 |
YJL099W |
Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane |
|
CHS6 |
4932 |
YJR033C |
Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate |
|
RAV1 |
4932 |
YKL190W |
Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 |
Null mutant is viable, Li+ and Na+ sensitive, cnb1 fks1 and cnb1 vma3 double mutants are inviable |
CNB1 |
4932 |
YKR020W |
Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p |
Null: small critical cell size |
VPS51 |
4932 |
YLR039C |
Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes |
defective in the transcription of both ribosomal protein genes and ribosomal RNA |
RIC1 |
4932 |
YLR262C |
GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 |
Null mutant is viable, temperature sensitive; suppressed by ssd1 and imh1 mutations |
YPT6 |
4932 |
YLR330W |
Protein involved in export from the Golgi to plasma membrane; involved in chitin biosynthesis through its role in Chs3p localization; interacts with Arf1p, Bch1p, Fmp50p, Bud7p, and Chs6p |
|
CHS5 |
4932 |
YMR004W |
Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole |
MVP1 was identified as a multicopy suppressor of dominant-negative vps1 mutations, as well as an extragenic suppressor of a temperature-sensitive pma1 mutation (sop gene) |
MVP1 |
4932 |
YMR307W |
Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor |
|
GAS1 |
4932 |
YNL041C |
Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
|
COG6 |
4932 |
YNL271C |
Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
Null mutant is viable, bni1 bnr1 double deletion mutants are temperature sensitive and are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate at the restrictive temperature; rho1 bni1 double mutants exhibit synthetic lethality |
BNI1 |
4932 |
YNR030W |
Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation |
|
ALG12 |
4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
4932 |
YOL018C |
Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation |
Null mutant is viable in SEY6210, exhibits endocytosis defect and loss of Kex2p |
TLG2 |
4932 |
YOR070C |
Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion |
Null mutant is viable and shows no phenotype |
GYP1 |
4932 |
YPL240C |
Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |