| 4932 |
YAL011W |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae |
|
SWC3 |
| 4932 |
YBR164C |
Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
|
ARL1 |
| 4932 |
YDL074C |
E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain |
null mutant is sensitive to brefeldin A |
BRE1 |
| 4932 |
YDR334W |
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Null mutant is viable and shows no growth defects; swr1 rat8-2 and swr1 rsc9-1double mutants has a slow growth phenotype; SWR1 is a partial High copy suppressor of pse1-1 kap123 |
SWR1 |
| 4932 |
YDR485C |
Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS72 |
| 4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
| 4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
| 4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
| 4932 |
YHR012W |
Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval |
Defective for sorting of soluble hydrolases to the vacuole. Mislocalisation of the vacuolar hydrolase sorting receptor Vps10p. |
VPS29 |
| 4932 |
YJL004C |
Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation |
Null mutant is viable. sys1 ypt6 double mutant displays enhanced defects in vacuolar sorting and cell growth |
SYS1 |
| 4932 |
YJL081C |
Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes |
|
ARP4 |
| 4932 |
YJR033C |
Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate |
|
RAV1 |
| 4932 |
YJR073C |
Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis |
Null mutant is viable, temperature sensitive in the presence of monomethylethanolamine, exhibits an inositol secretion phenotype |
OPI3 |
| 4932 |
YKL190W |
Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 |
Null mutant is viable, Li+ and Na+ sensitive, cnb1 fks1 and cnb1 vma3 double mutants are inviable |
CNB1 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YML041C |
Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS71 |
| 4932 |
YMR004W |
Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole |
MVP1 was identified as a multicopy suppressor of dominant-negative vps1 mutations, as well as an extragenic suppressor of a temperature-sensitive pma1 mutation (sop gene) |
MVP1 |
| 4932 |
YMR307W |
Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor |
|
GAS1 |
| 4932 |
YNL107W |
Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain |
|
YAF9 |
| 4932 |
YOL006C |
Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination |
|
TOP1 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOL018C |
Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation |
Null mutant is viable in SEY6210, exhibits endocytosis defect and loss of Kex2p |
TLG2 |
| 4932 |
YOR070C |
Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion |
Null mutant is viable and shows no phenotype |
GYP1 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
