4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
4932 |
YBR231C |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Cold-sensitive; Benomyl hypersensitive; Latrunculin-A hypersensitive |
SWC5 |
4932 |
YDL040C |
Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing |
Null mutant is viable, has reduced acetyltransferase activity, derepressed silent mating type locus (HML) and fails to enter G0 |
NAT1 |
4932 |
YEL061C |
Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation |
Null mutant is viable; cin8 dyn1 and cin8 kip1 double deletion mutants are inviable |
CIN8 |
4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
4932 |
YER122C |
ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p |
|
GLO3 |
4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
4932 |
YHR013C |
Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing |
|
ARD1 |
4932 |
YHR030C |
Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway |
Null mutant is viable but temperature sensitive. At elevated temperatures or in the presence of caffeine, mull mutants exhibit cell wall defects that result in cell lysis. Lysis is prevented by addition of 1M sorbitol. |
SLT2 |
4932 |
YHR090C |
Subunit of the NuA4 histone acetyltransferase complex that acetylates histone H4 and H2A; has similarity to the human tumor suppressor ING1 |
carbon source-, heat shock-, temperature-, and caffeine-sensitive, abnormal morphology, reduced histone H4 acetylation; BEM and RAD phenotypes; haploid yng2 mutants do not tolerate mutations in genes important for nonhomologous end joining repair yet remain proficient for homologous recombination. |
YNG2 |
4932 |
YHR191C |
Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
|
CTF8 |
4932 |
YHR200W |
Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein |
Null mutant is viable, exhibits a modest sensitivity to amino acid analogs and has increased steady-state levels of ubiquitin-protein conjugates |
RPN10 |
4932 |
YKL113C |
5- to 3- exonuclease, 5- flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family |
Null mutant demonstrates temperature-sensitive growth and sensitivity to UV light and methylmethane sulfonate. rad27 mutant cells are defective in Okazaki fragment maturation. |
RAD27 |
4932 |
YKR082W |
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p |
Null mutant is viable but grows slowly and is temperature-sensitive; at nonpermissive temperature, poly(A)+ RNA accumulates in nucleus (although nuclear import of karyophilic proteins is not blocked) and nuclear pores cluster; synthetically lethal with nup120 |
NUP133 |
4932 |
YLR320W |
Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks |
Null: Null phenotype in haploids of either mating type and diploid is extreme sensitivity to MMS or hydroxyurea, moderate sensitivity to gamma or UV irradiation. Diploid is very sensitive to camtothecin. Diploid is also sensitive to bleomycin.. |
MMS22 |
4932 |
YML094W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol; synthetically lethal with tub4-1 mutations |
GIM5 |
4932 |
YMR038C |
Copper chaperone for superoxide dismutase Sod1p, involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation |
Null mutant is viable, methionine and lysine auxotroph, pH and temperature sensitive; sensitive to superoxide generating drugs and light irradiation, exhibits diminution of calcineurin activity |
CCS1 |
4932 |
YNL153C |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
|
GIM3 |
4932 |
YNL298W |
Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p |
Null mutant is viable, possesses a cytokinesis defect; cla4 cln1 cln2 strains are inviable; cla4 ste20 double deletion mutants cannot maintain septin rings at the bud neck and and cannot undergo cytokinesis |
CLA4 |
4932 |
YNR051C |
Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A |
|
BRE5 |
4932 |
YOL115W |
Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase |
|
PAP2 |
4932 |
YOR080W |
Origin-binding F-box protein that forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication, involved in invasive and pseudohyphal growth |
Enhanced invasive growth in haploids; haploid budding pattern becomes polar. |
DIA2 |
4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |