| 4932 |
YAL011W |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae |
|
SWC3 |
| 4932 |
YAL012W |
Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine |
Null mutant is viable, cysteine auxotroph |
CYS3 |
| 4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
| 4932 |
YBR103W |
WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus |
Null mutant is viable, exhibits increased telomeric silencing |
SIF2 |
| 4932 |
YBR164C |
Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
|
ARL1 |
| 4932 |
YBR231C |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Cold-sensitive; Benomyl hypersensitive; Latrunculin-A hypersensitive |
SWC5 |
| 4932 |
YDL095W |
Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt2p, can instead interact with Pmt3p in some conditions; target for new antifungals |
Null mutant is viable but shows decrease by 40-50% of in vivo protein O-mannosylation; pmt1 pmt2 double mutant shows severe growth defect but residual O-mannosylation activity; the pmt1 pmt2 pmt3 pmt4 quadruple mutant is inviable |
PMT1 |
| 4932 |
YDL100C |
ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress |
Null: YDL100c gene disruption results in sensitivity to As(III), As(V), Co(II) and Cu(II). |
GET3 |
| 4932 |
YDL226C |
ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p |
|
GCS1 |
| 4932 |
YDR485C |
Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS72 |
| 4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
| 4932 |
YGL054C |
Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon |
Null mutant is viable but exhibits defects in sporulation (diploids) and bud site selection (haploids). Null mutants also retain the bud site selection marker, Axl2p, in the ER and exhibit slow recovery from selective to rich media. |
ERV14 |
| 4932 |
YJL004C |
Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation |
Null mutant is viable. sys1 ypt6 double mutant displays enhanced defects in vacuolar sorting and cell growth |
SYS1 |
| 4932 |
YJL056C |
Zinc-regulated transcription factor, binds to zinc-responsive promoter elements to induce transcription of certain genes in the presence of zinc; regulates its own transcription; contains seven zinc-finger domains |
High level expression of ZRT1 and ZRT2 in both zinc-limited and zinc-replete cells |
ZAP1 |
| 4932 |
YJR033C |
Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate |
|
RAV1 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YLR370C |
Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches |
|
ARC18 |
| 4932 |
YML121W |
Cytoplasmic GTP binding protein and negative regulator, with homolog Gtr2p, of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport; has homology to human RagA and RagB |
Null mutant is viable but grows slowly, is cold-sensitive, and has defects in phosphate uptake |
GTR1 |
| 4932 |
YMR073C |
Putative protein of unknown function; proposed to be involved in resistance to carboplatin and cisplatin; shares similarity to a human cytochrome oxidoreductase; null mutant displays increased levels of spontaneous Rad52 foci |
|
IRC21 |
| 4932 |
YMR161W |
Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Hdj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ |
|
HLJ1 |
| 4932 |
YMR202W |
C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis |
synthetic lethal with vma2. |
ERG2 |
| 4932 |
YMR216C |
SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 |
Slow growth; Decreased in vivo phosphorylation of npl3p |
SKY1 |
| 4932 |
YNL199C |
Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p |
Null mutant is viable and has partial growth defect on glucose-containing media |
GCR2 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOR002W |
Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease |
Null mutant is viable and defective in protein glycosylation. |
ALG6 |
