| 4932 |
YBR164C |
Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
|
ARL1 |
| 4932 |
YBR231C |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Cold-sensitive; Benomyl hypersensitive; Latrunculin-A hypersensitive |
SWC5 |
| 4932 |
YDL074C |
E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on K4 and K79), contains RING finger domain |
null mutant is sensitive to brefeldin A |
BRE1 |
| 4932 |
YDL220C |
Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping |
|
CDC13 |
| 4932 |
YDR334W |
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Null mutant is viable and shows no growth defects; swr1 rat8-2 and swr1 rsc9-1double mutants has a slow growth phenotype; SWR1 is a partial High copy suppressor of pse1-1 kap123 |
SWR1 |
| 4932 |
YDR485C |
Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS72 |
| 4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
| 4932 |
YER111C |
DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair |
Null mutant is viable, deficient in homothallic switching, and temperature sensitive |
SWI4 |
| 4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
| 4932 |
YGR105W |
Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also cannot grow on glycerol or at pH 7.5 |
VMA21 |
| 4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
| 4932 |
YKL113C |
5- to 3- exonuclease, 5- flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family |
Null mutant demonstrates temperature-sensitive growth and sensitivity to UV light and methylmethane sulfonate. rad27 mutant cells are defective in Okazaki fragment maturation. |
RAD27 |
| 4932 |
YKL190W |
Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 |
Null mutant is viable, Li+ and Na+ sensitive, cnb1 fks1 and cnb1 vma3 double mutants are inviable |
CNB1 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YLR110C |
Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor |
Null mutant is viable and shows decrease in mating efficiency and defect in agglutination |
CCW12 |
| 4932 |
YLR262C |
GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 |
Null mutant is viable, temperature sensitive; suppressed by ssd1 and imh1 mutations |
YPT6 |
| 4932 |
YLR268W |
R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog |
null mutant is cold and heat sensitive. Defective in ER to Golgi transport. |
SEC22 |
| 4932 |
YML041C |
Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS71 |
| 4932 |
YMR004W |
Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole |
MVP1 was identified as a multicopy suppressor of dominant-negative vps1 mutations, as well as an extragenic suppressor of a temperature-sensitive pma1 mutation (sop gene) |
MVP1 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOL018C |
Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation |
Null mutant is viable in SEY6210, exhibits endocytosis defect and loss of Kex2p |
TLG2 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
| 4932 |
YPL174C |
Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) |
Null mutant is viable but exhibits slow growth and defects in partitioning into daughter cells. |
NIP100 |
| 4932 |
YPL240C |
Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |
