| 4932 |
YAL011W |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae |
|
SWC3 |
| 4932 |
YBR231C |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Cold-sensitive; Benomyl hypersensitive; Latrunculin-A hypersensitive |
SWC5 |
| 4932 |
YCL016C |
Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance |
benomyl sensitive and defective in sister chromatid cohesion |
DCC1 |
| 4932 |
YDR334W |
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Null mutant is viable and shows no growth defects; swr1 rat8-2 and swr1 rsc9-1double mutants has a slow growth phenotype; SWR1 is a partial High copy suppressor of pse1-1 kap123 |
SWR1 |
| 4932 |
YDR388W |
Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin |
Null mutant is viable but exhibits reduced viability upon starvation |
RVS167 |
| 4932 |
YDR485C |
Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS72 |
| 4932 |
YEL061C |
Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation |
Null mutant is viable; cin8 dyn1 and cin8 kip1 double deletion mutants are inviable |
CIN8 |
| 4932 |
YER016W |
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally |
Null mutant is viable, causes cold sensitivity, benomyl supersensitivity, aberrant microtubule morphology. During mitosis in bim1 mutants, the nucleus fails to move to the mother-bud neck. |
BIM1 |
| 4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
| 4932 |
YGR105W |
Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also cannot grow on glycerol or at pH 7.5 |
VMA21 |
| 4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
| 4932 |
YHR030C |
Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway |
Null mutant is viable but temperature sensitive. At elevated temperatures or in the presence of caffeine, mull mutants exhibit cell wall defects that result in cell lysis. Lysis is prevented by addition of 1M sorbitol. |
SLT2 |
| 4932 |
YHR191C |
Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
|
CTF8 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YML041C |
Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS71 |
| 4932 |
YML094W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol; synthetically lethal with tub4-1 mutations |
GIM5 |
| 4932 |
YMR078C |
Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint |
Null mutant is viable, exhibits increased level of spontaneous mitotic recombination, slow growth, and cold sensitivity |
CTF18 |
| 4932 |
YNL153C |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
|
GIM3 |
| 4932 |
YNL271C |
Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
Null mutant is viable, bni1 bnr1 double deletion mutants are temperature sensitive and are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate at the restrictive temperature; rho1 bni1 double mutants exhibit synthetic lethality |
BNI1 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YPL031C |
Cyclin-dependent kinase, with ten cyclin partners; involved in environmental stress response; in phosphate-rich conditions, Pho85p-Pho80p complex phosphorylates Pho4p which in turn represses PHO5 |
|
PHO85 |
| 4932 |
YPL240C |
Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |
| 4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |
| 4932 |
YPR141C |
Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate |
Null mutant is viable. Mutations in KAR3 are semidominant and cause pleiotropic effects affecting both mitosis and meiosis. kar3 mutations prevent karyogamy (nuclear fusion). |
KAR3 |
