4932 |
YBR010W |
One of two identical histone H3 proteins (see also HHT2); core histone required for chromatin assembly, involved in heterochromatin-mediated telomeric and HM silencing; regulated by acetylation, methylation, and mitotic phosphorylation |
|
HHT1 |
4932 |
YCL016C |
Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance |
benomyl sensitive and defective in sister chromatid cohesion |
DCC1 |
4932 |
YDL042C |
Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and the rDNA locus; negatively regulates initiation of DNA replication |
Null mutant is viable; sir2 mutations suppress mitotic and meiotic intra- and interchromosomal rDNA recombination (10-15 fold). RAD52 and RAD50 are dispensable for basal level rDNA exchange in SIR2 but are required for increased exchange in sir2 |
SIR2 |
4932 |
YDR150W |
Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex |
Null mutant is viable; num1-disrupted strains contain many budded cells with two nuclei in mother cell, and haploid num1 strains tend to diploidize during mitosis |
NUM1 |
4932 |
YEL042W |
Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate |
|
GDA1 |
4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
4932 |
YGR105W |
Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also cannot grow on glycerol or at pH 7.5 |
VMA21 |
4932 |
YGR106C |
Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar memebrane |
|
YGR106C |
4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
4932 |
YLR039C |
Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes |
defective in the transcription of both ribosomal protein genes and ribosomal RNA |
RIC1 |
4932 |
YLR110C |
Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor |
Null mutant is viable and shows decrease in mating efficiency and defect in agglutination |
CCW12 |
4932 |
YLR262C |
GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 |
Null mutant is viable, temperature sensitive; suppressed by ssd1 and imh1 mutations |
YPT6 |
4932 |
YNL107W |
Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain |
|
YAF9 |
4932 |
YNL271C |
Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
Null mutant is viable, bni1 bnr1 double deletion mutants are temperature sensitive and are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate at the restrictive temperature; rho1 bni1 double mutants exhibit synthetic lethality |
BNI1 |
4932 |
YNL330C |
Histone deacetylase; regulates transcription and silencing |
Null mutant is viable and shows reduced potassium dependency, mating defects, hypersensitivity to cycloheximide, and constitutive derepression of acid phosphatase; mutant epistasis analysis indicates that RPD3 acts in the same pathway as UME4/SIN3; homozygous mutant diploid is defective in sporulation and recombination |
RPD3 |
4932 |
YOL006C |
Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination |
|
TOP1 |
4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
4932 |
YOR326W |
One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle |
Null mutant is inviable. myo2-66 (E511K), a temperature-sensitive allele, accumulates secretory vesicles and exhibits defects in initiation of new buds and delocalized chitin. |
MYO2 |
4932 |
YPL051W |
GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor |
Null mutant is viable, displays cold-sensitive growth |
ARL3 |
4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
4932 |
YPL240C |
Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |