| 4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
| 4932 |
YDR388W |
Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin |
Null mutant is viable but exhibits reduced viability upon starvation |
RVS167 |
| 4932 |
YER016W |
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally |
Null mutant is viable, causes cold sensitivity, benomyl supersensitivity, aberrant microtubule morphology. During mitosis in bim1 mutants, the nucleus fails to move to the mother-bud neck. |
BIM1 |
| 4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
| 4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
| 4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
| 4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
| 4932 |
YGR105W |
Integral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) |
Null mutant is viable but grows slowly and exhibits increased calcium sensitivity. Null mutants also cannot grow on glycerol or at pH 7.5 |
VMA21 |
| 4932 |
YGR106C |
Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar memebrane |
|
YGR106C |
| 4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
| 4932 |
YJL115W |
Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed |
|
ASF1 |
| 4932 |
YKL113C |
5- to 3- exonuclease, 5- flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family |
Null mutant demonstrates temperature-sensitive growth and sensitivity to UV light and methylmethane sulfonate. rad27 mutant cells are defective in Okazaki fragment maturation. |
RAD27 |
| 4932 |
YLR110C |
Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor |
Null mutant is viable and shows decrease in mating efficiency and defect in agglutination |
CCW12 |
| 4932 |
YLR342W |
Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling |
Null mutant is viable, demonstrates slow growth, hypersensitivity to FK506 and cyclosporin A, sensitivity to echinocandin and a reduction in 1,3-beta-D-glucan synthase activity in vitro; sensitivity to papulacandin B |
FKS1 |
| 4932 |
YML032C |
Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis |
Null mutant is viable, radiation sensitive; rad52 rad27 double mutants are inviable, double strand break ends are excessively recessed in mutant, rad52 is rescued by rad50 spo13, but not spo13, and is classified as late recombination gene. Growth defects of mgs1 rad18 double mutants are suppressed by overexpression of Rad52.; Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies. |
RAD52 |
| 4932 |
YMR186W |
Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |
| 4932 |
YMR224C |
Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved |
Null mutant is viable, methyl methanesulfonate-sensitive and displays hyper-recombination in mitosis. mre11 is rescued by spo13 and epistatic to rad50s, suggesting it is an early recombination function. |
MRE11 |
| 4932 |
YMR307W |
Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor |
|
GAS1 |
| 4932 |
YNL271C |
Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
Null mutant is viable, bni1 bnr1 double deletion mutants are temperature sensitive and are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate at the restrictive temperature; rho1 bni1 double mutants exhibit synthetic lethality |
BNI1 |
| 4932 |
YNL298W |
Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p |
Null mutant is viable, possesses a cytokinesis defect; cla4 cln1 cln2 strains are inviable; cla4 ste20 double deletion mutants cannot maintain septin rings at the bud neck and and cannot undergo cytokinesis |
CLA4 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YOR326W |
One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle |
Null mutant is inviable. myo2-66 (E511K), a temperature-sensitive allele, accumulates secretory vesicles and exhibits defects in initiation of new buds and delocalized chitin. |
MYO2 |
| 4932 |
YPL240C |
Cytoplasmic chaperone (Hsp90 family) required for pheromone signaling and negative regulation of Hsf1p; docks with the mitochondrial import receptor Tom70p for preprotein delivery; interacts with co-chaperones Cns1p, Cpr6p, Cpr7p, and Sti1p |
Null mutant is viable at 25 degrees C; ability to grow at higher temperatures varies with gene copy number |
HSC82 |
| 4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |
