| 4932 |
YBL061C |
Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation |
Null mutant is viable, resistant to Calcofluor white, exhibits a reduction in cell wall chitin and chitin synthase III activity |
SKT5 |
| 4932 |
YBR023C |
Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan |
|
CHS3 |
| 4932 |
YBR234C |
Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches |
|
ARC40 |
| 4932 |
YCR009C |
Amphiphysin-like lipid raft protein; subunit of a complex (Rvs161p-Rvs167p) that regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress |
Null mutant is viable, rvs161 mutations result in a delocalization of the actin cytoskeleton, high salt sensitivity, random budding pattern in diploid cells, defects in endocytosis, and reduced viability upon starvation; rvs161 mutants exhibit synthetic lethality with sst2 mutants |
RVS161 |
| 4932 |
YDL029W |
Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity |
cells with mutations in Arp2 and Arc15 are defective in mitochondrial movement. |
ARP2 |
| 4932 |
YDR388W |
Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin |
Null mutant is viable but exhibits reduced viability upon starvation |
RVS167 |
| 4932 |
YER111C |
DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair |
Null mutant is viable, deficient in homothallic switching, and temperature sensitive |
SWI4 |
| 4932 |
YGR229C |
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity |
Null mutant is viable, shows osmotic sensitivity, sensitivity to cercosporamide, resistance to zymolase; temperature sensitive mutant arrests at S phase with small buds |
SMI1 |
| 4932 |
YHR030C |
Serine/threonine MAP kinase involved in regulating the maintenance of cell wall integrity and progression through the cell cycle; regulated by the PKC1-mediated signaling pathway |
Null mutant is viable but temperature sensitive. At elevated temperatures or in the presence of caffeine, mull mutants exhibit cell wall defects that result in cell lysis. Lysis is prevented by addition of 1M sorbitol. |
SLT2 |
| 4932 |
YHR142W |
Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p export from the ER |
|
CHS7 |
| 4932 |
YJL095W |
Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p |
Null mutants are temperature-sensitive and exhibit cell lysis, which can be rescued by 1M sorbitol; null mutants grow very poorly even at the permissive temperature. Some dominant alleles suppress a pkc1 null mutant. |
BCK1 |
| 4932 |
YJL099W |
Member of the Chs5p-Arf1p-binding proteins (ChAPs), a group of 4 related, interacting proteins (Bch1p, Fmp50p, Bud7p, Chs6p) that mediate export of specific cargo proteins, including chitin synthase Chs3p, from the Golgi to plasma membrane |
|
CHS6 |
| 4932 |
YJR118C |
Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth |
|
ILM1 |
| 4932 |
YKL079W |
Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins |
high copy suppressor of myosin |
SMY1 |
| 4932 |
YKL190W |
Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1 |
Null mutant is viable, Li+ and Na+ sensitive, cnb1 fks1 and cnb1 vma3 double mutants are inviable |
CNB1 |
| 4932 |
YLR110C |
Cell wall mannoprotein, mutants are defective in mating and agglutination, expression is downregulated by alpha-factor |
Null mutant is viable and shows decrease in mating efficiency and defect in agglutination |
CCW12 |
| 4932 |
YLR342W |
Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling |
Null mutant is viable, demonstrates slow growth, hypersensitivity to FK506 and cyclosporin A, sensitivity to echinocandin and a reduction in 1,3-beta-D-glucan synthase activity in vitro; sensitivity to papulacandin B |
FKS1 |
| 4932 |
YMR307W |
Beta-1,3-glucanosyltransferase, required for cell wall assembly; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor |
|
GAS1 |
| 4932 |
YNL271C |
Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 |
Null mutant is viable, bni1 bnr1 double deletion mutants are temperature sensitive and are deficient in bud emergence, exhibit a random distribution of cortical actin patches and often become multinucleate at the restrictive temperature; rho1 bni1 double mutants exhibit synthetic lethality |
BNI1 |
| 4932 |
YNL294C |
Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH |
Null: Affected in sporulation and invasive growth. Other phenotypes: Alkaline sensitivity |
RIM21 |
| 4932 |
YNL322C |
Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor |
Null mutant is viable, exhibits reduction in cell wall (1--6)-beta-glucan |
KRE1 |
| 4932 |
YNR051C |
Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A |
|
BRE5 |
| 4932 |
YOR326W |
One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle |
Null mutant is inviable. myo2-66 (E511K), a temperature-sensitive allele, accumulates secretory vesicles and exhibits defects in initiation of new buds and delocalized chitin. |
MYO2 |
| 4932 |
YPL031C |
Cyclin-dependent kinase, with ten cyclin partners; involved in environmental stress response; in phosphate-rich conditions, Pho85p-Pho80p complex phosphorylates Pho4p which in turn represses PHO5 |
|
PHO85 |
| 4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
