| 4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |
| 4932 |
YAL024C |
Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP |
lethal at low temperature (8 degrees C) |
LTE1 |
| 4932 |
YCL016C |
Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance |
benomyl sensitive and defective in sister chromatid cohesion |
DCC1 |
| 4932 |
YCR066W |
Protein involved in postreplication repair; binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; forms heterodimer with Rad6p; contains RING-finger motif |
Radiation-sensitive. mgs1 exhibits a synergistic growth defect with rad18. Growth defects of mgs1 rad18 double mutants are suppressed by a mutation in SRS2 or by overexpression of Rad52.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD18 |
| 4932 |
YDL013W |
Protein containing a RING finger domain that interacts with Slx8p; mutant phenotypes and genetic interactions suggest a role in sumoylation and in genome stability |
null is synthetically lethal with sgs1 null |
SLX5 |
| 4932 |
YDL020C |
Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses |
Null mutant is viable, exhibits synthetic interactions with sen3, sun1, and cdc28-1N |
RPN4 |
| 4932 |
YDR369C |
Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling |
X-ray sensitive, spores inviable, xrs2 is rescued by spo13 and is epistatic to rad52 |
XRS2 |
| 4932 |
YER083C |
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function |
null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate |
GET2 |
| 4932 |
YER095W |
Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein |
Null mutant is viable; accumulates meiosis-specific double strand breaks at a recombination hotspot and reduces the formation of physical recombinants and processed double strand breaks with long heterogeneous tails; rad51 mutants are also defective for X-ray damage repair and gene conversions; rad51 rad27 mutants are inviable.; Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies. |
RAD51 |
| 4932 |
YGL020C |
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance |
Null: Required for spore wall formation, but not IME1 induction or nuclear division |
GET1 |
| 4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
| 4932 |
YHR191C |
Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
|
CTF8 |
| 4932 |
YHR200W |
Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein |
Null mutant is viable, exhibits a modest sensitivity to amino acid analogs and has increased steady-state levels of ubiquitin-protein conjugates |
RPN10 |
| 4932 |
YJR043C |
Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p |
Null mutant is viable but is cold-sensitive, hydroxyurea-sensitive, defective for induced mutagenesis, synthetic lethal with pol3, pol30 and pol31 |
POL32 |
| 4932 |
YKL113C |
5- to 3- exonuclease, 5- flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family |
Null mutant demonstrates temperature-sensitive growth and sensitivity to UV light and methylmethane sulfonate. rad27 mutant cells are defective in Okazaki fragment maturation. |
RAD27 |
| 4932 |
YML032C |
Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis |
Null mutant is viable, radiation sensitive; rad52 rad27 double mutants are inviable, double strand break ends are excessively recessed in mutant, rad52 is rescued by rad50 spo13, but not spo13, and is classified as late recombination gene. Growth defects of mgs1 rad18 double mutants are suppressed by overexpression of Rad52.; Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies. |
RAD52 |
| 4932 |
YMR078C |
Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint |
Null mutant is viable, exhibits increased level of spontaneous mitotic recombination, slow growth, and cold sensitivity |
CTF18 |
| 4932 |
YMR224C |
Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved |
Null mutant is viable, methyl methanesulfonate-sensitive and displays hyper-recombination in mitosis. mre11 is rescued by spo13 and epistatic to rad50s, suggesting it is an early recombination function. |
MRE11 |
| 4932 |
YNL273W |
Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase |
|
TOF1 |
| 4932 |
YNR052C |
RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3- to 5- mRNA deadenylation |
Mutant is resistant to glucose derepression, temperature-sensitive, and unable to sporulate and contains reduced amounts of reserve carbohydrates |
POP2 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOR080W |
Origin-binding F-box protein that forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication, involved in invasive and pseudohyphal growth |
Enhanced invasive growth in haploids; haploid budding pattern becomes polar. |
DIA2 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YPL055C |
Protein of unknown function; null mutant forms abnormally large cells |
Null: large cell size. Other phenotypes: synthetic lethal with swi4 |
LGE1 |
| 4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |
