| 4932 |
YBR231C |
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Cold-sensitive; Benomyl hypersensitive; Latrunculin-A hypersensitive |
SWC5 |
| 4932 |
YCL016C |
Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance |
benomyl sensitive and defective in sister chromatid cohesion |
DCC1 |
| 4932 |
YDR334W |
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
Null: Null mutant is viable and shows no growth defects; swr1 rat8-2 and swr1 rsc9-1double mutants has a slow growth phenotype; SWR1 is a partial High copy suppressor of pse1-1 kap123 |
SWR1 |
| 4932 |
YDR359C |
Component of the NuA4 histone acetyltransferase complex |
|
EAF1 |
| 4932 |
YEL003W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, sensitive to anti-microtubule drugs benomyl and nocadazole; synthetically lethal with tub4-1 mutations |
GIM4 |
| 4932 |
YEL061C |
Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation |
Null mutant is viable; cin8 dyn1 and cin8 kip1 double deletion mutants are inviable |
CIN8 |
| 4932 |
YER016W |
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally |
Null mutant is viable, causes cold sensitivity, benomyl supersensitivity, aberrant microtubule morphology. During mitosis in bim1 mutants, the nucleus fails to move to the mother-bud neck. |
BIM1 |
| 4932 |
YGL127C |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits |
Null mutant is viable, shows 10-fold increase in recombination. soh1 mutants were originally identified as suppressors of hyperrecombination hpr1 mutants. Soh1p may functionally interact with components of the RNA polymerase II complex as suggested from the synthetic lethality observed in soh1 rpb delta 104, soh1 rpb2, and soh1 sua7 double mutants. |
SOH1 |
| 4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
| 4932 |
YLR055C |
Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters |
Null mutant is viable, no growth defects, exhibits suppression of Ty insertion mutations, defects in Ty transcription |
SPT8 |
| 4932 |
YLR085C |
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A |
|
ARP6 |
| 4932 |
YLR200W |
Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin |
|
YKE2 |
| 4932 |
YML041C |
Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting |
Null mutant secretes CPY. |
VPS71 |
| 4932 |
YMR263W |
Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance |
|
SAP30 |
| 4932 |
YNL097C |
Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; C-terminus has similarity to human candidate tumor suppressor p33(ING1) |
Null mutant is viable but shows constitutive PHO5 expression |
PHO23 |
| 4932 |
YNL107W |
Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain |
|
YAF9 |
| 4932 |
YNL153C |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
|
GIM3 |
| 4932 |
YOL004W |
Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity |
inviable, reduced potassium dependency |
SIN3 |
| 4932 |
YOL012C |
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin |
Null mutant is viable at 28C; high copy suppressor of histone H4 point mutant affecting nucleosome structure |
HTZ1 |
| 4932 |
YOL115W |
Catalytic subunit of TRAMP (Trf4/Pap2p-Mtr4p-Air1p/2p), a nuclear poly (A) polymerase complex involved in RNA quality control; catalyzes polyadenylation of unmodified tRNAs, and snoRNA and rRNA precursors; disputed role as a DNA polymerase |
|
PAP2 |
| 4932 |
YOR244W |
Histone acetyltransferase catalytic subunit of the native multisubunit complex (NuA4) that acetylates four conserved internal lysines of histone H4 N-terminal tail; required for cell cycle progression |
|
ESA1 |
| 4932 |
YOR349W |
Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl |
Null mutant is viable, exhibits cold sensitivity for viability; defect in nuclear migration and nuclear fusion, supersensitivity to benomyl and nocodozole |
CIN1 |
| 4932 |
YPL008W |
Conserved nuclear protein required to establish sister-chromatid pairing during S-phase, probable DNA helicase with similarity to human BACH1, which associates with tumor suppressor BRCA1; associates with acetyltransferase Ctf7p |
Null mutant is viable, ts mutants mis-segregate chromosomes at permissive temperature leading to increased rate of mitotic chromosome loss, at non-permissive temperature ts mutants transiently arrest as large-budded cells with G(sub)2 DNA content and short spindle |
CHL1 |
| 4932 |
YPR135W |
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion |
Null mutant is viable but shows increase in the rate of mitotic chromosome loss, increased mitotic recombination, shift toward cells with G2 DNA content, and large budded cells with the nucleus in the bud neck; shows synthetic interactions with rad52, pol1, rad9, and esr1 |
CTF4 |
| 4932 |
YPR141C |
Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate |
Null mutant is viable. Mutations in KAR3 are semidominant and cause pleiotropic effects affecting both mitosis and meiosis. kar3 mutations prevent karyogamy (nuclear fusion). |
KAR3 |
