4932 |
YOR144C |
Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; involved in homologous recombination-mediated DNA repair |
|
ELG1 |
4932 |
YOR080W |
Origin-binding F-box protein that forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication, involved in invasive and pseudohyphal growth |
Enhanced invasive growth in haploids; haploid budding pattern becomes polar. |
DIA2 |
4932 |
YOR026W |
Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p |
|
BUB3 |
4932 |
YNR052C |
RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3- to 5- mRNA deadenylation |
Mutant is resistant to glucose derepression, temperature-sensitive, and unable to sporulate and contains reduced amounts of reserve carbohydrates |
POP2 |
4932 |
YNL273W |
Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase |
|
TOF1 |
4932 |
YNL153C |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
|
GIM3 |
4932 |
YMR198W |
Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p |
Null mutant is viable but is defective in both karyogamy and chromosome maintenance and does not show proper localization of Kar3p to microtubule-associated structures |
CIK1 |
4932 |
YMR078C |
Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint |
Null mutant is viable, exhibits increased level of spontaneous mitotic recombination, slow growth, and cold sensitivity |
CTF18 |
4932 |
YMR048W |
Protein required for accurate chromosome segregation during meiosis |
Null: missegregates chromosomes in meiosis |
CSM3 |
4932 |
YML094W |
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it |
Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol; synthetically lethal with tub4-1 mutations |
GIM5 |
4932 |
YLR320W |
Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks |
Null: Null phenotype in haploids of either mating type and diploid is extreme sensitivity to MMS or hydroxyurea, moderate sensitivity to gamma or UV irradiation. Diploid is very sensitive to camtothecin. Diploid is also sensitive to bleomycin.. |
MMS22 |
4932 |
YLR200W |
Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin |
|
YKE2 |
4932 |
YLL002W |
Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase, acetylates H3-K56; plays a role in regulation of Ty1 transposition |
Mutant exhibits abnormal mitochondrial morphology and slight growth defect in dextrose; insertion/truncation at amino acid 332 yields sensitivity to diepoxybutane and to mitomycin C; increase in Ty1 transposition |
RTT109 |
4932 |
YJL115W |
Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed |
|
ASF1 |
4932 |
YHR200W |
Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein |
Null mutant is viable, exhibits a modest sensitivity to amino acid analogs and has increased steady-state levels of ubiquitin-protein conjugates |
RPN10 |
4932 |
YHR191C |
Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion |
|
CTF8 |
4932 |
YGR078C |
Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
Null mutant is viable, benomyl sensitive, cold sensitive, microtubules disassemble at 14 degrees celsius, pac10 mutants exhibit synthetic lethality with tub4-1, cin8, cin1, pac2 and rbl2 mutants |
PAC10 |
4932 |
YGL058W |
Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (with Rad18p), sporulation, telomere silencing, and ubiquitin-mediated N-end rule protein degradation (with Ubr1p) |
Radiation sensitive. Defective for postreplication repair, repression of retrotransposition, meiotic gene conversion and sporulation. Mutations in srs2 suppress rad6 radiation-sensitivity but not the sporulation defect. rad6 forms recombination intermediates. mgs1 is synthetic lethal with rad6.; Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. |
RAD6 |
4932 |
YDR439W |
Protein involved in rDNA silencing; positively charged coiled-coil protein with limited similarity to myosin |
loses rDNA silencing |
LRS4 |
4932 |
YDL013W |
Protein containing a RING finger domain that interacts with Slx8p; mutant phenotypes and genetic interactions suggest a role in sumoylation and in genome stability |
null is synthetically lethal with sgs1 null |
SLX5 |
4932 |
YCR086W |
Nucleolar protein that forms a complex with Lrs4p which binds Mam1p at kinetochores during meiosis I to mediate accurate chromosome segregation, may be involved in premeiotic DNA replication; possible role in telomere maintenance |
|
CSM1 |
4932 |
YCL061C |
S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint |
Null: sensitive to hydroxyurea; replication checkpoint defective; slower DNA replication than wild type; partial loss of silencing at telomeres and HM loci; synthetic lethal with rad9 null, rad53-21, and mec1-21. |
MRC1 |
4932 |
YAR002W |
Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance |
|
NUP60 |
4932 |
YAL024C |
Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP |
lethal at low temperature (8 degrees C) |
LTE1 |
4932 |
YAL021C |
Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
reduced levels of ADH2 expression under both glucose and ethanol growth conditions; temperature sensitive growth on nonfermentative medium |
CCR4 |